General Biotechnology
My Genes: The Blueprint of Life
 
- GBI-BE-101 My Genes: The Blueprint of Life, 6 groups of 4-5 students    
           
 

The key biotechnology molecule that has probably had the most media coverage is DNA (also referred to as genes and genomes) that supplies the blueprint for each individual. To grip your students imagination and introduce them to DNA have them see their very own genome. This exercise allows students to comprehend previously mysterious DNA and its function as the blueprint of life.
The kit is designed to give each student experience on how DNA is isolated by scientists from tiny amounts of samples, such as cheek cells. Students collect their own cheek cells, which are first broken open in a solution to release proteins and cellular components. Proteins and cellular materials are then removed by the action of a protease, which essentially “eats” the proteins and releases the genetic material (DNA). The DNA is precipitated with alcohol and visualized as a suspension of long, white strands.
The kit is suitable for junior school grade levels to college and university levels and even those adults and professionals seeking an understanding of molecular basis of life and genomic DNA.

Supplied with components needed for hands-on experimentation for six workstations of 4-5 students or 24-30 students. Supplied with Teacher's Guide and separate Student's Guides.

   
Biotechnology in Your Mouth
Unlocking the Mysteries of Genetic Material
DNA Fingerprinting
DNA Strands Revealed
Microorganisms In Our Environment
How Clean Is Our Water
  Microbial Studies
Bacterial Culture & Growth Study
Bacterial Gram Staining
Antibiotic Sensitivity & Bacteria Screening
Isolation & Characterization of Bacteria
  Molecular and Genomic Studies
Bacterial Conjugation
Viral Transduction
Isolate Your Own Genomic DNA
Introduction to Agarose Electrophoresis
Polymerase Chain Reaction (PCR)
Purification of a Gene
DNA Restriction Digestion Analysis
DNA Ligation
Bacterial Transformation
Plasmid Isolation (Alkaline Lysis)
Plasmid Isolation (Solid Particle)
Expression of a Recombinant Protein
Genetic Defect Correction with Bacterial Transformation
Mutation Detection & Analysis
Southern Blot Analysis
Onion or Bacterial Genomic DNA Isolation
Nucleic Acid Quantification
  Physical Properties of Proteins
Physical Properties of Proteins
Hydrophobic & Hydrophilic Proteins
Biodiversity Study & Biomass Analysis
Assays for Protein Quantification
Enzyme Analysis
Protein Structure Analysis
Protein Degradation Study
  Protein Purification
Protein Purification from Tissue
Recombinant Protein Purification
Hydrophobic Chromatography
Size Exclusion Chromatography
Ion Exchange Chromatography
Affinity Chromatography
Protein Fractionation
Subcellular Fractionation & Protein Distribution
  Protein Electrophoresis
Protein Folding Study
Subcellular Fractionation & Protein Distribution
Protein Structure Analysis
Protein Electrophoresis
Protein Degradation Study
Zymogram: Study of an Active Enzyme with Electrophoresis
  Immunotechnology Studies
Antigen-Antibody Interactions
Dot Blot Analysis
Western Blot Analysis
Immunoquantification Technique (ELISA)
Quantitative Precipitin Assay (QPA)
Immunoprecipitation Technique
Immunoaffinity Chromatography
  Miscellaneous Biotechniques
Fatty Acids, Lipids & Membranes
Electroelution
Dialysis
Protein Labeling
Protein Cross-linking
RNA Isolation