General Biotechnology
My Genes: The Blueprint of Life
Biotechnology in Your Mouth
Unlocking the Mysteries of Genetic Material
 
- GBI-BE-103 Unlocking the Mysteries of Genetic Material, 6 groups of 4-5 students    
           
 

Molecular biology and genetic engineering are important skills used in the scientific world that allow for the modification and manipulation of DNA to generate proteins that have been used as drugs or novel food sources. The techniques of molecular biology and genetic engineering are crucial tools used in many of today’s biotechnology fields, including forensic science and scientific research.
This kit unlocks the mysteries of genetic materials by introducing students to the concepts of genes, transferable DNA (plasmids) and bacteria as living factories for the production of important proteins. Students will understand the relationship between genes and proteins, by transforming a gene, encoding a light emitting protein, into bacteria to produce glowing green bacteria (see figure). In addition to expressing the light emitting protein, the trait for drug resistance is also conferred on the bacteria, allowing students to learn about antibiotic selection.

Supplied with components needed for hands-on experimentation for six workstations of 4-5 students or 24-30 students. Supplied with Teacher's Guide and separate Student's Guides.

           
DNA Fingerprinting
DNA Strands Revealed
Microorganisms In Our Environment
How Clean Is Our Water
  Microbial Studies
Bacterial Culture & Growth Study
Bacterial Gram Staining
Antibiotic Sensitivity & Bacteria Screening
Isolation & Characterization of Bacteria
  Molecular and Genomic Studies
Bacterial Conjugation
Viral Transduction
Isolate Your Own Genomic DNA
Introduction to Agarose Electrophoresis
Polymerase Chain Reaction (PCR)
Purification of a Gene
DNA Restriction Digestion Analysis
DNA Ligation
Bacterial Transformation
Plasmid Isolation (Alkaline Lysis)
Plasmid Isolation (Solid Particle)
Expression of a Recombinant Protein
Genetic Defect Correction with Bacterial Transformation
Mutation Detection & Analysis
Southern Blot Analysis
Onion or Bacterial Genomic DNA Isolation
Nucleic Acid Quantification
  Physical Properties of Proteins
Physical Properties of Proteins
Hydrophobic & Hydrophilic Proteins
Biodiversity Study & Biomass Analysis
Assays for Protein Quantification
Enzyme Analysis
Protein Structure Analysis
Protein Degradation Study
  Protein Purification
Protein Purification from Tissue
Recombinant Protein Purification
Hydrophobic Chromatography
Size Exclusion Chromatography
Ion Exchange Chromatography
Affinity Chromatography
Protein Fractionation
Subcellular Fractionation & Protein Distribution
  Protein Electrophoresis
Protein Folding Study
Subcellular Fractionation & Protein Distribution
Protein Structure Analysis
Protein Electrophoresis
Protein Degradation Study
Zymogram: Study of an Active Enzyme with Electrophoresis
  Immunotechnology Studies
Antigen-Antibody Interactions
Dot Blot Analysis
Western Blot Analysis
Immunoquantification Technique (ELISA)
Quantitative Precipitin Assay (QPA)
Immunoprecipitation Technique
Immunoaffinity Chromatography
  Miscellaneous Biotechniques
Fatty Acids, Lipids & Membranes
Electroelution
Dialysis
Protein Labeling
Protein Cross-linking
RNA Isolation