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DNA Sequencing Service |
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Service |
Turnaround Time |
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Single Pass DNA Sequencing |
~ 4 working days |
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Type of DNA Samples |
Concentration & Volume/ Reaction |
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Purified PCR Product [90-250bp] |
10 ng/uL, min 10 uL per rxn in dH2O |
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Purified PCR Product [251-500bp] |
20 ng/uL, min 10 uL per rxn in dH2O |
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Purified PCR Product [>500bp] |
40 ng/uL, min 10 uL per rxn in dH2O |
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Purified Plasmid |
50 ng/uL, min 10 uL per rxn in dH2O |
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Purified M13 ssDNA |
50 ng/uL, min 10 uL per rxn in dH2O |
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Purified Genomic DNA |
400 ng/uL, min 20 uL per rxn in dH2O |
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Primer Walking [< 3kb] |
50 – 200 ng/uL, in 50 uL sdH2O |
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Primer Walking [> 3kb] |
Whole bacterial colony; or 50 – 200 ng/uL, in 50 uL sdH2O |
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Type of Universal Primers |
Sequence (5' - 3') |
Primer provided by customer |
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M13F (-20) |
5' GTAAAACGACGGCCAGT 3' |
10uM or 10pmol/uL, in 5uL dH2O |
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M13F (-29) |
5' CACGACGTTGTAAAACGAC 3' |
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M13F-pUC (-40) |
5' GTTTTCCCAGTCACGAC 3' |
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M13R (-20) |
5' GCGGATAACAATTTCACACAGG 3' |
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M13R (-24) |
5' GGAAACAGCTATGACCATG 3' |
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M13R-pUC (-26) |
5' CAGGAAACAGCTATGAC 3' |
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T7 |
5' AATACGACTCACTATAG 3' |
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T7 Promoter |
5' TAATACGACTCACTATAGGG 3' |
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T7 Terminator |
5' GCTAGTTATTGCTCAGCGG 3' |
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T3 |
5' ATTAACCCTCACTAAAG 3' |
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SP6 |
5' ATTTAGGTGACACTATAG 3' |
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BGH-rev |
5' CTAGAAGGCACAGTCGAGGC 3' |
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AOX1 Forward |
5' GACTGGTTCCAATTGACAAGC 3' |
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AOX1 Reverse |
5' GCAAATGGCATTCTGACATCC 3' |
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pBRrevBam |
5' GGTGATGTCGGCGATATAGG 3' |
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